Characterization of the endoplasmic reticulum morphogenic mutant of Arabidopsis thaliana, GFP-CXN
Title
Characterization of the endoplasmic reticulum morphogenic mutant of Arabidopsis thaliana, GFP-CXN
Subject
Life Sciences
Description
Two lines of Arabidopsis thaliana are studied in Dr Breeze’s lab. One is GFP-HDEL which is a control line of Arabidopsis, whilst the other is GFP-CXN a genetically modified line that overexpresses a domain of chaperone protein called calnexin in its endoplasmic reticulum (ER). This causes GFP-CXN to have a highly interconnected network of flattened, membrane-enclosed sacs in its ER known as cisternae. The cisternae have a crucial role in the modification of proteins.
It has been suggested in previous studies that whilst challenging the lines with the plant pathogen Pseudomonas syringae DC3000 the GFP-CXN line has been less susceptible to infection than the control potentially due to the ER’s highly cisternal morphology. However, to support this hypothesis further characterization of GFP-CXN is required.• The aim of my project was to further characterize the GFP-CXN line by investigating chloroplast efficiency/function during infection by measuring Fv/Fm, image ER morphology in a confocal microscope, compare bacterial susceptibility by completing a bacterial growth assay and investigate physiology by measuring and comparing root
elongation rate with the control.
It has been suggested in previous studies that whilst challenging the lines with the plant pathogen Pseudomonas syringae DC3000 the GFP-CXN line has been less susceptible to infection than the control potentially due to the ER’s highly cisternal morphology. However, to support this hypothesis further characterization of GFP-CXN is required.• The aim of my project was to further characterize the GFP-CXN line by investigating chloroplast efficiency/function during infection by measuring Fv/Fm, image ER morphology in a confocal microscope, compare bacterial susceptibility by completing a bacterial growth assay and investigate physiology by measuring and comparing root
elongation rate with the control.
Creator
Alexander Gale
Date
2022
Files
Collection
Citation
Alexander Gale, “Characterization of the endoplasmic reticulum morphogenic mutant of Arabidopsis thaliana, GFP-CXN,” URSS SHOWCASE, accessed November 2, 2024, https://urss.warwick.ac.uk/items/show/149.