Controlling polymer molecular weight to optimise DNA complexation in PolyHIPlex-based systems
Title
Controlling polymer molecular weight to optimise DNA complexation in PolyHIPlex-based systems
Subject
Chemistry
Creator
Nicole Bernstein
Contributor
Supervisors: Dr. Santhosh Kalash Rajendrakumar and Professor Sebastien Perrier (Perrier Group)
Abstract
Reversible Addition-Fragmentation Chain Transfer (RAFT) polymerisation enables precise control over polymer molecular weight and dispersity, allowing the synthesis of structurally controlled polymers for gene delivery applications. This project investigated how polymer molecular weight influences DNA complexation in PolyHIPlex-based systems, which offer an alternative to lipid nanoparticles for nucleic acid delivery. The polymers were synthesised using controlled RAFT conditions and characterised via NMR and GPC, confirming successful polymerisation and tunable molecular weights. These findings support the development of more efficient and adaptable PolyHIPlex systems for DNA delivery.
Files
Collection
Citation
Nicole Bernstein, “Controlling polymer molecular weight to optimise DNA complexation in PolyHIPlex-based systems,” URSS SHOWCASE, accessed November 1, 2025, https://urss.warwick.ac.uk/items/show/1027.