2D vs 3D Models of Nasopharyngeal Carcinoma: Why 3D? An RNA Sequencing Analysis
Title
2D vs 3D Models of Nasopharyngeal Carcinoma: Why 3D? An RNA Sequencing Analysis
Subject
Life Sciences
Description
Cancer Genomics
Creator
Madeleine Andrea
Date
2025
Contributor
Morgan Barton , Chris Dawson , Lawrence Young , Hannah E. Bridgewater
Abstract
There are major differences in the regulation of genes in 2D and 3D cell cultures. Through RNA sequencing analysis, this project inspects some of these differences in Epstein-Barr Virus (EBV) positive Nasopharyngeal Carcinoma (NPC) cultures, particularly demonstrating a downregulation in key regulators of the cell cycle such as the Anaphase Promoting Complex (APC/C) and Origin Recognition Complex (ORC) in the 3D models. This is evidence that 3D models are more representative of tumour microenvironments and therefore supports their use in pre clinical studies for improved downstream analysis.
Files
Collection
Citation
Madeleine Andrea, “2D vs 3D Models of Nasopharyngeal Carcinoma: Why 3D? An RNA Sequencing Analysis,” URSS SHOWCASE, accessed November 3, 2025, https://urss.warwick.ac.uk/items/show/887.