1 10 1 https://urss.warwick.ac.uk/files/original/375ae1260dd66264df8fa2dc091b2bb7.pdf 73194dfe29c9fd8677e95254e2481aba Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Science, Engineering and Medicine Academic Poster An academic poster Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Characterization of the endoplasmic reticulum morphogenic mutant of Arabidopsis thaliana, GFP-CXN Subject The topic of the resource Life Sciences Description An account of the resource Two lines of Arabidopsis thaliana are studied in Dr Breeze’s lab. One is GFP-HDEL which is a control line of Arabidopsis, whilst the other is GFP-CXN a genetically modified line that overexpresses a domain of chaperone protein called calnexin in its endoplasmic reticulum (ER). This causes GFP-CXN to have a highly interconnected network of flattened, membrane-enclosed sacs in its ER known as cisternae. The cisternae have a crucial role in the modification of proteins. It has been suggested in previous studies that whilst challenging the lines with the plant pathogen Pseudomonas syringae DC3000 the GFP-CXN line has been less susceptible to infection than the control potentially due to the ER’s highly cisternal morphology. However, to support this hypothesis further characterization of GFP-CXN is required.• The aim of my project was to further characterize the GFP-CXN line by investigating chloroplast efficiency/function during infection by measuring Fv/Fm, image ER morphology in a confocal microscope, compare bacterial susceptibility by completing a bacterial growth assay and investigate physiology by measuring and comparing root elongation rate with the control. Creator An entity primarily responsible for making the resource Alexander Gale Date A point or period of time associated with an event in the lifecycle of the resource 2022 Arabidopsis thaliana Endoplasmic GFP-CXN Morphogenic