1 10 2 https://urss.warwick.ac.uk/files/original/fd003ca3e90b9847b7a584f97d1c460a.pdf 536358b16844687393c259d4cbc9ce65 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Science, Engineering and Medicine Academic Poster An academic poster Abstract The abstract of the paper Brassica oleracea is a common family of crop plants including Cabbage, Broccoli, Cauliflower, Kale and Brussels sprout. Brassicas are susceptible to a host of pests and disease such as Black rot. Immune signal pathways, consisting of essential phosphorylation events, remain poorly studied and unclear in many crop plants such as Brassica oleracea. To identify the most phosphoproteins, high-throughput mass spectrometry-based phosphoproteomics was used. The research group’s standard protocol involves a single TiO2 enrichment step, but still misses some essential phosphoproteins. To investigate whether increasing the number of enrichments improves the phosphoprotein identification, three enrichment steps were used and over 1000 phosphoproteins from 1370 unique peptides were identified. The 3 phosopho-peptide enrichment steps generally increased the peptide yield by 25% compared to the standard 1 enrichment. The difference in physiochemical properties between samples was caused by a higher abundance of more hydrophilic and highly phosphorylated serine peptides in the 1st enrichment. This observed enhancement in phosphopeptide yield will enable a significant increase in phosphoprotein identification and demonstrate more novel phosphorylation events related to immune pathways. Characterising these mechanisms will provide direct experimental evidence for resistance genes and will play an important role in annotating these genes. This annotation can be used to breed disease resistance crops and increase food security. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Is Peptide Yield enhanced by repeated Phospho-enrichments in Brassicas? Subject The topic of the resource Life Sciences Description An account of the resource Brassica oleracea is a common family of crop plants susceptible to disease. In this work the research groups' Mass Spectrometry-based Phosphoproteomics method was improved by increasing TiO2 enrichment steps. This enabled the research group to identify key phosphoproteins involved in immune signal transduction. Creator An entity primarily responsible for making the resource Sean Connors Date A point or period of time associated with an event in the lifecycle of the resource 2021 Brassica Oleracea Brassicas Immunity Mass Spectroscopy PAMPs Phosoproteomics Plant Immunity Proteomics https://urss.warwick.ac.uk/files/original/f4fc79d5b9e4575a064f110caabf8fb3.pdf ed1c4f06f62b408f5a7a28f6715d5b6c Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Science, Engineering and Medicine Presentation Meta Tags Meta tags Tag 1 :The function of TrPM2 on Neuronal Cell ---if it signal to PGE2; Tag 2 :determine the specific roles of TRPA1, TRPV1, and TRPM2 in mediating the response to PGE2. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource The function of TrPM2 on Neuronal Cell Creator An entity primarily responsible for making the resource Yuemeng Chen Subject The topic of the resource Life Sciences Date A point or period of time associated with an event in the lifecycle of the resource 2024 Contributor An entity responsible for making contributions to the resource Xuming Zhang anti-inflammatory Disease Immunity inflammation neuronal Cell rPM2